The inclusion body washed with % deoxycholic acid sodium salt and dissolved with 0.% N-lauroyl sarcosine sodium was recovered by the way of dialysis,then the protein was purified with Glutathione Sepharose TM~(B) and its purity was above 8%.
The inclusion body washed with % deoxycholic acid sodium salt and dissolved with 0.% N-lauroyl sarcosine sodium was recovered by the way of dialysis,then the protein was purified with Glutathione Sepharose TM~(B) and its purity was above 8%.
以包涵体形式存在的重组蛋白以%的脱氧胆酸钠洗涤、0.%的N-十二烷基肌氨酸钠溶解、透析复性、Glutathione Sepharose TMB纯化后,纯度达8%以上。