7kb exon6 to exon7 DNA sequence which is separated by PCR as Short Arm(SA),were used to construct HPRT gene knock out vector.
7kb exon6 to exon7 DNA sequence which is separated by PCR as Short Arm(SA),were used to construct HPRT gene knock out vector.
根据已知大鼠HPRT基因的外显子序列,从大鼠HPRT基因BAC中用酶切和PCR方法分别分离得到用于构建基因敲除载体的3。