To study the suitable method for cattle oviduct simple epithelium cells culture, the epithelium cells were isolated by cutting and 0.25% trypsinization, the exponential phase of growth cells vigor and growth velocity was determined by MTT method, the viab
To study the suitable method for cattle oviduct simple epithelium cells culture, the epithelium cells were isolated by cutting and 0.25% trypsinization, the exponential phase of growth cells vigor and growth velocity was determined by MTT method, the viab
摘要为探讨适用于黄牛输卵管单层上皮细胞的培养方法，采用机械剪取及0.25%胰酶消化的方法分离获得上皮细胞，取对数生长期细胞进行MTT比色实验检测细胞活力和生长速度；台盼蓝排斥试验检测活细胞数。