Genomic DNA and mRNA were isolated from Aspergillus niger, and the high efficient primers was designed by species similarity. With the methods of PCR and RT-PCR respectively, the fragments of the DNA and cDNA of beta-glucosidase gene were obtained.
Genomic DNA and mRNA were isolated from Aspergillus niger, and the high efficient primers was designed by species similarity. With the methods of PCR and RT-PCR respectively, the fragments of the DNA and cDNA of beta-glucosidase gene were obtained.
以黑曲霉基因组DNA、mRNA为模板,利用种属相似性设计了一对引物,采用PCR与RT-PCR技术得到β-葡萄糖苷酶基因的DNA和cDNA全序列.