The recombinant E. colt expressing IFN-α2a was cultured in 500L fermenterunder the condition of cascade controlling dissolved oxygen content. The result showed alinear relation between cell density and culture time (b= 6. 6668, r= 0. 9963, P<0.01).
The recombinant E. colt expressing IFN-α2a was cultured in 500L fermenterunder the condition of cascade controlling dissolved oxygen content. The result showed alinear relation between cell density and culture time (b= 6. 6668, r= 0. 9963, P<0.01).
用500L大型发酵罐级联控制溶氧培养表达α－2a干扰素的大肠杆菌，获得了菌体生长与培养时间呈直线关系的结果（b＝6．668，r＝0．9963，P＜0．01）。