Based on the reported sequences of Swine/CH/IMH/0 ,a pair of primers to amplify the ABC gene was designed. The total RNA was extracted from China strain of porcine teschen virus,and the ABC gene was amplified by RT-PCR.
Based on the reported sequences of Swine/CH/IMH/0 ,a pair of primers to amplify the ABC gene was designed. The total RNA was extracted from China strain of porcine teschen virus,and the ABC gene was amplified by RT-PCR.
根据已报道的Swine/CH/IMH/0 基因序列设计对引物,从已分离的中国株捷申病毒中提取总RNA,经RT-PCR扩增得到 ABC基因.