Before transformation, alfalfa suspension cultures were treated with 0.mol/L mannitol for h, and washed with 0.mol/L CaCl .H O for two times.
Before transformation, alfalfa suspension cultures were treated with 0.mol/L mannitol for h, and washed with 0.mol/L CaCl .H O for two times.
悬浮培养物在用于转化之前，用0mol／L甘露醇处理h，然后用0mol／LCaCl·HO洗涤两次。