Amplification performed in a Whatman Biometra tgradient AmpGene DNA Thermal Cycler was programmed for min at 9 , cycles of min at 9 , min for -STS or for 0 -SCAR, min at 7 , and a final extention at 7 for0 min after the last cycle.
Amplification performed in a Whatman Biometra tgradient AmpGene DNA Thermal Cycler was programmed for min at 9 , cycles of min at 9 , min for -STS or for 0 -SCAR, min at 7 , and a final extention at 7 for0 min after the last cycle.
9℃，min，℃-STS min或0℃-SCAR min，7℃，min， 0个循环； 0min，7℃延伸；