When the demi-embryos pre-freezing cultured for . 0, . ～. 0 and . 0 hours were frozen in 0. M sucrose/PBS solution containing 0% glycerol (supple mented with 0% dextran CT-00), the survival rates were 0% (0/), 8. 8% (/) and 7 % (9/),respectively.
When the demi-embryos pre-freezing cultured for . 0, . ～. 0 and . 0 hours were frozen in 0. M sucrose/PBS solution containing 0% glycerol (supple mented with 0% dextran CT-00), the survival rates were 0% (0/), 8. 8% (/) and 7 % (9/),respectively.
半胚冷冻前培养 ．0、．～．0、．0小时后，以添加0％而聚糖的0％甘油＋0．M蔗糖／PBS液冷冻，分别获得了0％（0／）、8．8％（／）、7．0％（9／）的存活率。