The technology of high cell density culture for this recombinant was established. In repeated culture for three times,the average cell density A 600 nm was 58,dry cell weight was 18.0 g/L and rIL 2 expression was 42.4% of total cell protein.
The technology of high cell density culture for this recombinant was established. In repeated culture for three times,the average cell density A 600 nm was 58,dry cell weight was 18.0 g/L and rIL 2 expression was 42.4% of total cell protein.
通过三批重复实验，确定了rIL－2工程菌高密度培养方案，平均菌密度A600nm值为58，菌干重为18．0g／L，rIL－2表达占菌体总蛋白的42．4％。